Catalogue number
PKA-072
Synonyms
Protein
Interacting With PRKCA 1, PRKCABP, Protein Kinase C-Alpha-Binding Protein, Protein
Interacting With C Kinase 1, Protein Kinase C, Alpha Binding Protein, Protein
Interacting With PRKCA, PRKCA-Binding Protein, PICK, PRKCA-binding protein.
Introduction
Protein Interacting with PRKCA 1, also known as PICK1 includes a PDZ
domain, through which it interacts with protein kinase C, alpha (PRKCA). PICK1
performs as an adaptor which binds to and arranges the subcellular localization
of various membrane proteins. PICK1 interacts with multiple glutamate receptor
subtypes, monoamine plasma membrane transporters, in addition to non-voltage
gated sodium channels, and might target PRKCA to these membrane proteins and therefore
regulates their distribution as well as function. PICK1 has also been found to perform
as an anchoring protein which particularly targets PRKCA to mitochondria in
a ligand-specific manner.
Description
PICK1 Human Recombinant produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 438 amino acids (1-415 a.a) and having a molecular
mass of 49.0kDa.
PICK1 is fused to a 23
amino acid His-tag at N-terminus & purified by proprietary chromatographic
techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
PICK1 protein solution (0.25mg/ml) containing Phosphate
buffered saline (pH7.4), 30% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH
SSGLVPRGSH MGSMFADLDY
DIEEDKLGIP TVPGKVTLQK DAQNLIGISI GGGAQYCPCL YIVQVFDNTP AALDGTVAAG DEITGVNGRS
IKGKTKVEVA KMIQEVKGEV TIHYNKLQAD PKQGMSLDIV LKKVKHRLVE NMSSGTADAL GLSRAILCND
GLVKRLEELE RTAELYKGMT EHTKNLLRAF YELSQTHRAF GDVFSVIGVR EPQPAASEAF VKFADAHRSI
EKFGIRLLKT IKPMLTDLNT YLNKAIPDTR LTIKKYLDVK FEYLSYCLKV KEMDDEEYSC IALGEPLYRV
STGNYEYRLI LRCRQEARAR FSQMRKDVLE KMELLDQKHV QDIVFQLQRL VSTMSKYYND CYAVLRDADV
FPIEVDLAHT TLAYGLNQEE FTDGEEEEEE EDTAAGEPSR DTRGAAGPLD KGGSWCDS.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
