Catalogue number
CYT-890
Synonyms
MPL Ligand, THCYT1, MPLLG, TPO, ML, Thrombopoietin
Nirs Variant 1, MKCSF, Thrombopoietin, Myeloproliferative Leukemia Virus
Oncogene Ligand, Megakaryocyte Growth and Development Factor, Megakaryocyte
Colony-Stimulating Factor, C-Mpl Ligand, MGDF, Megakaryocyte Stimulating
Factor, Prepro-Thrombopoietin.
Introduction
| Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidney which regulates the production of platelets by the bone marrow. TPO stimulates the production as well as differentiation of megakaryocytes, the bone marrow cells which fragment into large numbers of platelets. |
Description
TPO produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 340 amino acids (22-353aa.) and
having a molecular mass of 36.5kDa. (Molecular size on SDS-PAGE will appear at
approximately 40-57KDa).
TPO is expressed with an 8 amino acid His tag at C-Terminus
and purified by proprietary chromatographic techniques..
Source
Sf9,
Insect cell.
Physical Appearance
Sterile Filtered clear solution.
Formulation
TPO protein solution (0.25mg/ml) contains phosphate
buffered saline (pH7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 85% as determined by SDS-PAGE.
Amino acid sequence
SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNELPNRTSG
LLETNFTASA RTTGSGLLKW QQGFRAKIPG LLNQTSRSLD QIPGYLNRIH ELLNGTRGLF PGPSRRTLGA PDISSGTSDT GSLPPNLQPG YSPSPTHPPT GQYTLFPLPP TLPTPVVQLH PLLPDPSAPT PTPTSPLLNT SYTHSQNLSQ EGLEHHHHHH.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
