Catalogue number
PRO-2137
Synonyms
39S ribosomal protein L2, mitochondrial , CGI-22,
MRP-L14, RPML14, L2mt, MRP-L2, CGI-22.
Introduction
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and aid in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) comprised of a small 28S subunit and a large 39S subunit. Among different species, the proteins comprising the mitoribosome vary greatly in sequence, and sometimes in biochemical properties, thus preventing simple recognition by sequence homology. Mitochondrial Ribosomal Protein L2 (MRPL2) is a 39S subunit protein which is a member of the EcoL2 ribosomal protein family.
Description
MRPL2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 142 amino acids (84-202 a.a) and having a molecular mass of 15.5kDa.
MRPL2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
MRPL2 protein solution (0.25mg/ml) containing 20mM
Phosphate buffer (pH 8.0), 1mM EDTA, 50% glycerol and 2mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSGRDHTGR IRVHGIGGGH KQRYRMIDFL RFRPEETKSG PFEEKVIQVR
YDPCRSADIA LVAGGSRKRW IIATENMQAG DTILNSNHIG RMAVAAREGD AHPLGALPVG TLINNVESEP GR.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
