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dCas9 C-terminal Cloning Vector

品牌：Abmgood

货号：K014

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dCas9 C-terminal Cloning Vector

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	Print Version
Description	This Cas9 Double Mutant (dCas9) has changes at amino acid positions D10A and H840A which completely inactivate both the nuclease and nickase activities. With a target-specific guide RNA (sgRNA), it can bind to a specific region of DNA without cutting it. This vector provides an in-frame downstream multiple cloning site, allowing users to attach any protein of interest to the C-terminus of dCas9. The resulting construct will also carry a C-terminal nuclear localization signal.
Product Application	Direct non-viral plasmid transfection for immediate expression Package into Lentiviral particles for high efficiency transduction and stably integrated expression
Species	N/A
Vector	pLenti-EF1a-dCas9-Cloning
Bacterial Selection	Kanamycin
Format	Plasmid
Appearance	Liquid
Storage	-20°C or below
Shelf Life	1 year (when at -20°C or below in a non-frost free freezer)
Shipping	Shipped at ambient temperature
Additional Materials	- CRISPR Genomic Cleavage Detection Kit (G932) - sgRNA CRISPR Lentivector Control (K018)
Quality Control	Restriction Enzyme Digest and Sequencing
Caution	This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Note	abm's sgRNAs are designed for use with Cas9 Nuclease only. Please inquire for custom sgRNAs for purposes other than genome editing (e.g. CRISPRa). For CRISPR-Cas9 system applications in genome regulation, please see our comprehensive collection of Cas9 mutant proteins (Nickase, Null mutant).
Disclaimer	1. The end user acknowledges that the Materials provided under abm’s Material Transfer Agreement (MTA) does not grant a license for commercial use or imply any ownership rights of any intellectual property rights relating to the Materials.