Description
The ViPrimePLUS Rotavirus A RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Rotavirus A genome in clinical samples (e.g. stool), contaminated food samples and environmental water samples.
Rotavirus is a double-stranded RNA virus that is subdivided into group A to G. Group A, B and C are the most relevant to humans, causing severe acute diarrhoea. Particularly in developing countries, rotavirus gastroenteritis is the major cause of mortality and morbidity among young children. Due to the viral stability in the environment, transmission occurs via ingesting contaminated food and water. The characteristics of the disease are vomiting and watery diarrhoea for 3-8 days, i.e. self-limiting, fever and abdominal pain. Primary infection does not provide complete immunity, however repeat infection tends to be less severe. Vaccination is currently available to cope with the viral infections.
ViPrimePLUS Rotavirus A RT-qPCR Kit is intended for research use only.
Kit Component
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RotaV A Primers and Probe Mix (RotaV A PPM)
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Positive Control
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Internal Extraction Control Primers and Probe Mix (IEC PPM)
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Internal Extraction Control (IEC DNA)
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Nuclease Free Water
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Template Preparation Buffer
Product Specification
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Technology
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Taqman probe-based real-time PCR assay
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Type of Nucleic Acid Kit
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RNA
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Kit Storage
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Shipped in room temperature, store at -20℃ on arrival
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Sample Material
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• Clinical samples (e.g. stool)
• Food samples
• Environmental water samples
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Gene Target
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Non-structural protein 5 (NSP5) encoding gene
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Detection Limit
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10 to <100 copies per reaction
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Sensitivity & Specificity
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Ct value 28±3 with probability of 95%
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Controls Included
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• Internal Extraction Control (IEC) as inhibition control
• Positive control
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Channels
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• FAM channels detect pathogen amplicons
• VIC/HEX channel detects IEC amplicons
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Ordering Information
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Catalog No
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Description
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Pack Size
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QM5071
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ViPrimePLUS Rotavirus A RT-qPCR Kit
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150 reactions
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Download
Manual
ViPrimePLUS Rotavirus A RT-qPCR Kit
