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Concentration
10-40u/μl
5'…GGCCNNNN↓NGGCC…3'
3'…CCGGN↑NNNNCCGG…5'

Reaction Conditions 
1X Buffer V2
10mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl₂, 50mM NaCl, and 100μg/ml BSA. Incubate at 50°C.

Storage Buffer
10mM Tris-HCl (pH 7.6), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 10-fold overdigestion with SfiI, 70% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 40u of SfiI for 16 hours at 50°C.
Supplied with 10X Buffer V2, 10X Buffer UB and Viva Buffer A. (Diluent)
*Blocked by overlapping dcm-methylation (C^mCWGG): GGCCWGGNNGGCC 
*Not blocked by overlapping dam-methylation (C^mCWGG): GGCCNNNNNGGCCWGG.

Ordering Information

Downloads
Manual
SfiI

Publication
This Product Has Been Used In:
Chuah, L.O., Shamila-Syuhada, A.K., Liong, M.T., Rosma, A., Thong, K.L., Rusul, G. (2016) ) Physio-chemical, microbiological properties of tempoyak and molecular characterisation of lactic acid bacteria isolated from tempoyak